Protein Method Blocking Buffers
Bioworld ELISA Reaction Buffer, 50mM Carbonate-Bicarbonate, 1 L
Product DescriptionELISA reaction buffer has a 50 mM concentration of carbonate-bicarbonate and a pH level of 9.6. It contains 1.59 g Na2CO3, 2.93 g NaHCO3, and dissolved in 1 liter of deionized water.Carbonate/bicarbonate buffer at a pH greater than 9 is used for the attachment of an antigen or of an antibody onto an ELISA plate. Direct coating of ELISA is an antigen immobilization technique, in which antigens are directly attached to the plate by passive adsorption. Most but not all proteins bind tightly to the polystyrene surface of microplates in alkaline conditions. Indirect coating of ELISA is another antigen immobilization technique in which antigen-specific antibodies are adsorbed onto the plastic, which in turn binds and immobilizes the antigen upon incubation with the antigen sample. Both techniques typically use the same buffer.
LICOR Intercept T20 (TBS) Protein-Free Antibody Diluent (500 mL)
Intercept T20 (TBS) Protein-Free Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects. The diluent contains Intercept (TBS) Protein-Free Blocking Buffer in Tris-buffered saline (TBS) preformulated with Tween 20. This diluent can be used where animal-based products are prohibited. Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation. Intercept T20 (TBS) Protein-Free Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
Abcam He x amethonium bromide, nicotinic receptor blocker, 1G
MW 362.19 Da. Ganglionic blocker; antagonist at α₃-containing nicotinic acetylcholine receptors (autonomic ganglia).
The product is subject to the following: Abcam Restricted Use Statement
LICOR Intercept T20 (PBS) Antibody Diluent (10 x 500 mL)
Intercept T20 (PBS) Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects.The diluent contains Intercept (PBS) Blocking Buffer preformulated with Tween 20 for use in a phosphate-buffered saline (PBS) system. Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation. Intercept T20 (PBS) Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
Bioworld NAP- (Non-Animal Protein) Blocker, 1 L
NAP (Non Animal Protein)-BLOCKER provides improved assay sensitivity, clean background and high signal-to-background ratio. Produced from non-animal proteins that are free of biotin and other agents that interfere with immunoassays. Will not cross react with animal source antigens or primary and secondary antibodies. Supplied as a 4X solution, simply mix with water to obtain a 1X ready to use buffer for nitrocellulose and PVDF membranes. Suitable for up to 100 mini-gel blots using plastic bags for reduced volume. Usually Ships within 24 HoursAdvantage:Reduces risk of non-specific binding Lowers background Improves assay sensitivityApplication: Western blot, dot blot & ELISA experiments
Bioworld Casein Blocking Buffer with Fish Gelatin, 100 mL
Casein Fish Gelatin Blocking Buffer is a non-mammalian blocking solution that does not cross-react with mammalian antibodies and is suitable for ELISA assays. Blocking buffer is supplied in a 10X concentration of physiological saline buffer. Technical Information: Casein Blocker with Fish Gelatin is a casein-based blocking agent Includes 3 X 30 mL, 10X concentratePrepares 900ml of working solution1X working solution contains: 1% casein, 0.45% fish gelatin in PBS.
Surmodics Inc StabilCoat Immunoassay Stabilizer, 50mL
StabilCoat Immunoassay Stabilizer, and the 5X concentrate, effectively preserves the conformation and activity of dried proteins while simultaneously blocking the surface to reduce non-specific binding. The product can be used to stabilize antibodies, antigens or enzymes on an assortment of immunoassay components including polystyrene plates, tubes, glass, membranes, and filter paper.
AbD Serotec Block ACE, 20 X 4g
Block Ace is designed as a high-performance blocking reagent for use in immunological assays such as ELISA and Western blotting. It may also be used for dilution of antibodies and for washing procedures in the above assays.
Biotang Inc GELATIN- Blocking Buffer in PBS (with azide), 500 ml
GELATIN- Blocking Buffer in PBS (with azide), 500 ml.
Abcam Immunoassay Blocking (BSA Free), 50ML
Abcam’s Immunoassay Blocking (BSA Free) (ab171535) maintains the activity of antibodies and other biomolecules adsorbed or immobilized to solid substrates.
The product is subject to the following: Abcam Restricted Use Statement
LICOR Intercept (PBS) Blocking Buffer (10 x 500mL)
Intercept (PBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. Intercept (PBS) Blocking Buffer is the blocking buffer of choice for near-infrared fluorescent applications when using a phosphate-buffered saline (PBS) system. It contains no mammalian proteins for the lowest cross-reactivity with mammalian antibodies, it is an excellent choice for biotin-streptavidin detection and it is suitable for a wide range of applications, including Western blots, In-Cell Western™ assays, and protein arrays. Intercept (PBS) Blocking Buffer is a ready-to-use blocker formulation in PBS. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection. It is stored at 4 °C and is also available in kits with Immobilon-FL PVDF and nitrocellulose membranes.
Biotang Inc CASEIN- Blocking Buffer in PBS (with azide), 250 ml
CASEIN- Blocking Buffer in PBS (with azide), 250 ml.
Biolegend Tris-Buffered Saline 20X (1.0 M) w/ Tween 20
Tris-Buffered Saline 20X (1.0 M) w/ Tween 20 Apps: IHC; Size: 500 mL
Biotium TrueBlack WB Blocking Buffer Kit
The TrueBlack WB blocking buffer is a buffer system designed to achieve optimal specificity and sensitivity by blocking background from the non-specific interaction between the fluorophore-labeled antibodies and the membrane with cell lysates. For blocking and antibody dilution for 50 membranes.
FOCUS BIOMOLECULES Biochemical Reagents, MitoBloCK-10, TIMM44 blocker, CAS 394694-98-5, 98% by TLC/NMR (Conforms)
Biochemical Reagents, MitoBloCK-10, TIMM44 blocker, CAS 394694-98-5, 98% by TLC/NMR (Conforms), MitoBloCK-10 is a first in class TIMM44 blocker. Translocase of inner mitochondrial membrane 44 (TIMM44) is essential for maintaining function and integrity of mitochondria.1 It induced mitochondrial dysfunction and suppressed angiogenic activity in endothelial cells.1 Targeting TIMM44 by MitoBloCK-10 significantly inhibits bladder cancer cell growth in vitro and in vivo.2